Heidolph Kugelmeiers 3D Cell Culture Plates - Sphericalplate 5D (SP5D)
The spherical plate 5D (SP5D) is a 3D cell culture plate for the formation of uniform, standardized spheroids with high quality and yield. It allows the effortless scaling and translation into the diagnostic or clinical application. At the same time, the reproducibility of follow-up tests increases, since the experiments always start under identical initial conditions due to the low size variance of the cultivated spheroids.
The patented geometry with special coating allows the integration of every single cell into a 3D-agglomerate in a controlled way. This is how uniformly sized spheroids grow without the formation of necrotic cores. According to the size and scale of the experiments, you can choose between our 96-well plate, 24-well plate or our 6-well plate.
Guidance
For small batches, we suggest using the 24-well plate containing 12 wells with 750 microwells each. Here, you will be able to yield up to 9,000 uniform spheroids. Furthermore, you can comfortably carry out monolayer comparative studies in the remaining 12 wells of the same plate.
The 6-well plate is suitable for scale-up trials and bigger batches. You can expect a yield of 3,364 spheroids per well, thus a total yield of 20,184 spheroids per plate.
For small-scale experiments or standardized procedures, the standard 96-well format is suitable. One plate contains 12 strips, each with 8 wells, and each well holds 25 microwells, yielding a total harvest of up to 2,400 spheroids per plate.